This unnamed man was a clerk at a truck stop near Des Moines, Iowa.
During Jacob Frankenstein's search for Charlie Bradbury, he came to the truck stop that this man worked at and questioned him for information. The man insisted that he had not seen Charlie and Jacob then slit his throat with a knife.
While Jacob was cleaning his knife after the murder, hunter Dean Winchester entered the store and Jacob posed as the clerk to avoid suspicion. However, Dean spotted the Styne Family crest on Jacob's hand and looked in the security mirror where he saw the man's body lying on the ground. A scuffle ensued that ended with Jacob running one of his henchmen dead.