Scooby-Doo is a talking dog who is part of a group of "mystery hunters" called the Scooby Gang, along with Daphne Blake, Fred Jones, Velma Dinkley, and his best friend, Shaggy Rogers, in the Scooby Doo Universe. He appeared in Season 12 and Season 13 on television, as well as the season 13 Supernatural/Scooby-Doo, Where Are You! official crossover titled Scoobynatural where Sam and Dean Winchester, along with the angel Castiel, are transported into his world.
In The Magnificent Seven, Dean and Sam, along with Bobby Singer, tell Tamara and Isaac that they should fight the Seven Deadly Sins together. Isaac retorts, saying that this isn't Scooby-Doo and he doesn't want to work with the ones who opened the gates to Hell.
In Family Remains, Dean and Sam are at a house hunting what they believed was a ghost. As they attempt to get the family out of the house, little boy Danny Carter asks if they are like Scooby-Doo, to which Dean replies they are better.
In All Dogs Go To Heaven, Dean asks the skinwalker Lucky where his "little Scooby gang" is, referring to his pack of skinwalkers. Lucky responds by telling them they are laying low while they wait for the alpha skinwalker to come.
In Dog Dean Afternoon, Dean uses an Inuit animal communication spell to communicate with The Colonel. When Sam points out that the spell was causing him to act like a dog, Dean whimpers and then uses Scooby's iconic phrase, "r'uh r'oh!".
In Thinman, Dean and Sam are on a hunt when they see the Ghostfacers van outside of a restaurant. They go inside and see Ghostfacers' leaders Ed Zeddmore and Harry Spangler. Dean then tells them that they need to get into their "mystery machine," referring to their van, and leave before he shoots them in the knees.
In Form and Void, Crowley and Dean are searching Jenna Nickerson's house for Jenna and baby Amara, and Dean yells for Jenna. Crowley then warns him that they are basically telling Amara that they are coming,to which Dean replies by calling him Velma and that they aren't the Scooby gang, so he needs to shut up or leave. Crowley retorts by saying he's "way more of a Daphne."
In LOTUS, Dean and Sam go to a monastery looking for Lucifer, who had possessed an archbishop. As they reach the door, Dean tells Sam that, if this is in fact Lucifer, that they'll call the rest of the Scooby gang, referring to Crowley, Castiel, and Rowena MacLeod.
In Regarding Dean, while Dean is under Gideon Loughlin's memory erasing spell, he sits in his and Sam's hotel room while Rowena explains the spell and Dean's possibility of dying from it. Sam decides to turn the TV on to distract Dean, showing the episode of Scooby-Doo, Where Are You!, titled "Hassle in the Castle". Scooby is shown on the TV.
In The Rising Son, Dean, Sam, and Jack get a hotel room on their way back from North Cove, Washington to their bunker in Lebanon, Kansas. Jack sits down on one of the two beds and turns on the TV showing the Scooby-Doo, Where Are You! episode "That's Snow Ghost". Scooby is shown on the TV, until Dean walks over. He then proceeds to take the remote from Jack. He gets distracted watching it, but then turns the TV off and tells Jack he is on the couch.
In Scoobynatural, Dean and Sam are given a brand-new TV for free after believing they defeated a giant, living dinosaur plushy. When the TV starts to act odd, sending Sam and Dean into the Scooby-Doo, Where Are You! episode "A Night of Fright Is No Delight" where they team up with Daphne Blake, Fred Jones, Scooby-Doo, Shaggy Rogers, and Velma Dinkley to check out a haunted house owned by Colonel Sanders who named Scooby as a beneficiary in his will. Cosgood Creeps tells Scooby and the other beneficiaries that they will get a million dollars inheritance, but only if they survive staying the night there. However, when they discover a real dead body, Dean realizes that the episode they are in is not going the way it is supposed to, Cosgood being meant to be the villain. Meanwhile, Castiel returns to the bunker with the fruit from the tree of life for the spell to open a door to Apocalypse World, and is sucked in as well. Castiel catches up to the group, accidentally scaring them. When they split up to search the house, Castiel is paired with Scooby and Shaggy. When the ghost attacks the group, Shaggy is thrown through a window and falls off a balcony. When Scooby dives after him, Castiel does the same, grabbing Scooby's tail, who has caught Shaggy, and using his trenchcoat he slows their fall. Shaggy breaks his arm when they land, and Sam, Dean, and Castiel explain to them about ghosts, monsters, and even demons. After Fred concocts a plan to trap the ghost and it ends up trapping Scooby, Shaggy, and Castiel instead, they concoct another plan, trapping the ghost in a circle of salt. After discovering the ghost was a young child being used by a shady real estate developer to scare people into selling their property, the Winchesters and Castiel convince the ghost to pose as Cosgood so they can tell the gang that it was actually him just tricking them. They then say their goodbyes and Castiel is shown to have gained a fondness for Scooby and Shaggy. The ghost then sends them back to their world, and they discover that a item that was keeping the kid on earth and allowing the real estate developer to scare people had been planted in the TV and the plushy dinosaur. After freeing the ghost, they have the real estate developer arrested for tax fraud. When he says that he would have gotten away with it if it hadn't been for those meddling kids, Dean recognizes it as a phrase said at the end of most Scooby-Doo episodes, he attempts the ending where Scooby says his name into the camera, to which Castiel tells him he is not a talking dog.
In Mint Condition, Sam uses cleaning supplies inside of a Scooby-Doo themed lunch box, showing pictures of Scooby and Shaggy, to blow up a door. Dean later says that next year they will do Halloween right and even suggests multiple matching costume ideas to Sam, one of which being Scooby and Shaggy.
- Season 3
- The Magnificent Seven (mentioned only)
- Season 4
- Family Remains (mentioned only)
- Season 6
- All Dogs Go to Heaven (mentioned only)
- Season 8
- The Great Escapist (mentioned only)
- Season 9
- Season 11
- Form and Void (mentioned only)
- Season 12
- Season 13
- Season 14
- Mint Condition (picture and mentioned)