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This article is about a character appearing in the animated series, which is not canon. You may be looking for the live-action series counterpart, if any.
Madison was a character from Supernatural: The Animation.
Madison was the assistant of Mulligan, a lawyer working at the DA's office. One night, Mulligan was killed by what appears to be a wild dog, though Sam and Dean Winchester correctly deduced that it was a werewolf attack.
During interrogation, Madison reveals she was having problems with her ex-boyfriend, who often suspected there was something between her and Mulligan. Sam, having grown attached to her due to the similarities between Madison and Jessica, promised to protect Madison whatever the cost.
Problems arose when Madison herself transformed into a werewolf and killed Kurt. Dean wanted to kill her, but Sam chose to find an alternative. They agreed to hunt down the one responsible for biting Madison, but despite killing the werewolf, Madison remained unchanged.
Madison desperately wanted to kill herself out of grief, but Sam stopped her every time. Eventually, the brothers were forced to end her life when she attacked Dean. Sam was the one to shoot her.