The Fruit of the Tree of Life is the fruit taken from a Tree of Life in Syria.
As seen in the Scoobynatural, the fruit appears like a mangosteen with brownish skin. When crushed, however, the content of the fruit similar to red fig. Other than this, any special characteristics of the fruit are currently unknown.
After returning to the Men of Letters bunker, Castiel reveals that he has gotten a bag of the fruit. According to Castiel, he had to battle a clan of djinn guarding the tree for it. Castiel killed most of the djinn and bargained with the rest, possibly leaving Castiel married to the djinn clan's queen. When Castiel got sucked into Scooby-Doo, Where Are You!, he dropped the bag with the fruit and a few spilled out.
In a flashback it was revealed that somehow the Men of Letters came to know of the ritual needed to open a portal to another world. In 1925, after having somehow gathered all of the ingredients one of which was Fruit of the Tree of Life, rogue Men of Letters member Diego Avila performed the ritual in the Portsmouth, Rhode Island chapter house to bring the gods Yokoth and Glythur from their world to Earth in order to cleanse it and start over. Diego and his followers succeeded in opening the rift and summoning Yokoth who possessed Sandy Porter. Yokoth ate most of the Men of Letters involved, but the survivors managed to close the rift and bound Yokoth who could not be killed.
In 2018, after tracking the Seal of Solomon to the Portsmouth chapter house, the Winchesters inadvertently freed Yokoth. Yokoth later used ingredients stored in a hidden compartment to replicate the ritual to open a rift into her own world and bring Glythur into the Main Universe in the body of Dean Winchester. Sam arrived with Marco and Ophelia Avila before she could succeed. Dean managed to free himself and steal the Seal of Solomon which Ophelia used to close the rift. Before the rift closed, Yokoth was pulled back through by Glythur.
After getting all of the needed ingredients, Sam used the fruit along with the blood, Seal and Gabriel's grace to open a rift to Apocalypse World that Dean traveled through with Arthur Ketch. After Dean returned, the Winchesters could not repeat the ritual as Gabriel had departed and they had no more of his grace despite having everything they needed.
Awhile later, witch Rowena MacLeod repeated the ritual with Gabriel's grace, but he didn't have enough and the rift fizzled out. After capturing Lucifer, his grace was used to open the rift and keep it open continuously.
- Season 13
- While it's unknown if it has a similar history to real world Christianity. In Christianity the Tree of Life is a holy tree in the Garden of Eden that originally gave immortality. It was when Adam and Eve were expelled from the Garden, that the deprivation of the fruit of the tree began them to age.