Ellen and Jo

"I will always love you baby"- Ellen to Jo

Ellen and Jo Harvelle are mother and daughter. They also live and work together at the Roadhouse. They have a good relationship besides the fact that Jo wants to want, and Ellen won't let her. Eventually, Jo does get into hunting and Ellen joins her. Ellen strongly believes if her daughter is going to put herself in danger, she gonna be there to protect her. They are together till the end.

Background
Ellen and Jo have lived at the Roadhouse all their lives before they met Sam and Dean. Ellen's late husband Bill, was a hunter, which is why Ellen and Jo know so much about the supernatural. Then on a mission with John Winchester, Bill gets killed. After losing Bill, Ellen makes sure that Jo never gets near anything related to hunting. Although she doesn't let Jo walk around unprotected. Jo carries a knives with her so she can protect herself. Although, Jo still wants to go into the hunting lifestyle despite her mother's wishes.

Season 2
" Joanna Beth, this family has lost enough. Now I won't lose you too." -Ellen When the Winchesters come into their bar in"Everybody Laves a Clown", Ellen and Jo work together to capture the both of them. But they Ellen recognizes them and releases them. They explain to Sam and Dean about the Roadhouse and how they know about the supernatural. At the end of the episode, the boys come back to the Roadhoouse and drink with Ellen and Jo.

In "Simon Said" Jo is shown beating a Hunter at a shooting game in the Roadhouse. After he gives her the money they bet, Ellen walks up and proudly tells him he should check the high scores, which are all Jo.

Alternative Reality
In My Heart Will Go On, Ellen and Jo are still alive and hunting together.