Holy water

Other than Salt, Holy Water is another common tool hunters use. It is mainly used for fighting off demons. Its not until season 3, that holy water is used more often as there are more demons about. Most hunter carry about a silver bottle of holy water with them most of the time.

Holy water though, cannot defeat a demon, but it hurt and burn it. This allows hunter to interrogate demons for information.

How It's Created
Holy Water is created by chanting a latin chant over a source of water with a rosary in hand. Once the chant is complete, the rosary is dropped into the water, and it becomes holy water.

First Appearance
It first appear in Phantom Traveler with the first demon appearing on the show. A demon was making planes crash, so Sam and Dean hop on a plane to excercise the demon. Dean manages to sneak the holy water past security. They figure out it the co-pilot was possessed and trap him in the back. There they use the holy water on him, which causes the demon to be in pain and burns to appear.

Other Uses

 * Sprinklers
 * Piping
 * Torture
 * Bobby's panic room walls (soaked with holy water and salt)