Decapitation

Decapitation is the act of cutting off a being's head. It can be used to disable certain supernatural beings, such as vampires, shapeshifters, arachnes, Leviathans and even demons.

Vampires
Chopping off a vampire's head will kill it.

Ghouls
Complete removal of the head is lethal for a ghoul.

Arachnes
Beheading will kill an arachne.

Shapeshifters
Chopping off a shapeshifter's head will kill it, even their alpha.

Jefferson Starships
Cutting off a Jefferson Starship's head will kill it.

Leviathans
A Leviathan's head can be chopped off, which can be used to stop it temporarily, until it eventually reattaches itself.

Demons
A demon's vessel's head can be chopped off, but, through possession, they can move it and sow it back on.

Nachzehrers
A nachzehrer's head can be chopped off but it will remain animated unless a copper coin is placed in the mouth first. If a copper coin is in the nachzehrer's mouth before they are decapitated, they will be killed. Otherwise, the head can be put back on the nachzehrer's body and reattach itself.

Bisaans
Besaans can be killed by decapitation.

Humans
Being entirely mortal, humans can be killed through decapitation as well. Dean was able to kill Cuthbert Sinclair through decapitation with the First Blade.

Werewolves
Mary Winchester was able to kill a werewolf in 1980 through decapitation with a shotgun.

Appearances

 * Season 1
 * Nightmare (Human)
 * Dead Man's Blood (Vampires)


 * Season 2
 * Bloodlust (Vampires)


 * Season 3
 * Fresh Blood (Vampires)


 * Season 4
 * Jump the Shark (Ghoul)


 * Season 5
 * Free To Be You and Me (Vampire)
 * Fallen Idols (Deity)


 * Season 6
 * Live Free or Twihard (Vampires)
 * Family Matters (Vampires)
 * Caged Heat (Alpha Shapeshifter)
 * Unforgiven (Arachne)
 * My Heart Will Go On (Human)
 * Mommy Dearest (Jefferson Starships)


 * Season 7
 * Shut Up, Dr. Phil (Human)
 * Slash Fiction (Leviathan)
 * Out With The Old (Leviathan)
 * There Will Be Blood (Leviathan)
 * Survival of the Fittest (Leviathan)


 * Season 8
 * We Need To Talk About Kevin (Vampires)
 * Blood Brother (Vampires and Leviathans)
 * A Little Slice of Kevin (Leviathans)
 * Citizen Fang (Vampire)
 * As Time Goes By (Demon)
 * Freaks and Geeks (Vampires)
 * Taxi Driver (Vampires)
 * Season 9
 * Devil May Care (Vampire)
 * Blade Runners (Human and Vampires)
 * Alex Annie Alexis Ann (Vampires)
 * Bloodlines (Vampire)


 * Season 10
 * Ask Jeeves (Human)
 * Hibbing 911 (Vampires)
 * The Werther Project (Vampires and Leviathan hallucination)
 * Brother's Keeper (Vampires)


 * Season 11
 * Baby (Nachzehrer)
 * Don't You Forget About Me (Vampires)
 * The Chitters (Bisaans)


 * Season 12
 * Keep Calm and Carry On (Vampire)
 * Celebrating The Life Of Asa Fox (Werewolf)

Trivia

 * Dean Winchester has performed the most decapitations on the show. He has decapitated at least 50 people on-screen.