Alpha's blood



The Alpha Vampire's blood is one of the three bloods the Winchesters need to construct their anti-Leviathan weapon since the Alpha Vampire is the first Vampire, and thus a Father of Fallen Beasts.

After learning from Crowley that the Alpha Vampire is still alive, the Winchesters track him down to his residence. They ask the Alpha for his blood and try to convince him that the Leviathan are trying to wipe the Vampires out. The Alpha initially refuses, but after an assassination attempt by Edgar - which the Winchesters manage to foil - he agrees to give them some of his blood. He slits his wrist and bleeds into a goblet which he then gives to the Sam and Dean.